Hi there, I'm a member of the team at Google responsible for this dataset.
I don't see an artfact at exactly those coordinates, but is this nearby location what you are referring to:
tinyurl.com/4rpas73s
That appears to be a knife mark. This dataset was assembled from over 5000 tissue sections each cut at a thickness of just 30 nanometers using a device called an ultramicrotome. Tiny imperfections can develop in the diamond knife used by the ultramicrotome, which unfortunately can result in artifacts like that. Fortunately such artifacts are unlikely to affect the same location on multiple consecutive sections, and our segmentation algorithms are robust enough to segment through those locations.
By the way, one cool thing about the viewer, neuroglancer, is that you can just copy the URL to link directly to any particular view.
This dataset was collected using physical ~33nm-thick sections that were cut with a diamond knife and stored (and preserved) on a carbon tape before imaging with a multi-beam scanning electron microscope.
The latter. The tissue block is sliced first in the ATUM which collects the sections on tape. These are then mounted on silicon wafers, and imaged with the MultiSEM.
I don't see an artfact at exactly those coordinates, but is this nearby location what you are referring to:
tinyurl.com/4rpas73s
That appears to be a knife mark. This dataset was assembled from over 5000 tissue sections each cut at a thickness of just 30 nanometers using a device called an ultramicrotome. Tiny imperfections can develop in the diamond knife used by the ultramicrotome, which unfortunately can result in artifacts like that. Fortunately such artifacts are unlikely to affect the same location on multiple consecutive sections, and our segmentation algorithms are robust enough to segment through those locations.
By the way, one cool thing about the viewer, neuroglancer, is that you can just copy the URL to link directly to any particular view.